potential co-treatment effects of aataria multiflora, camellia sinensis extracts and bacterial lipopolysaccharide on skin wound model

Camellia sinensis and Zataria multiflora as the potent antioxidants and antiinflammations are crucial in the management of wound therapy. Lipopolysaccharide [LPS] was used for the proliferative potential on fibroblast cells and induction of inflammatory mediators

Objective: We examined the effects of LPS and herbal extracts combination in order to identify their mechanisms of action in fibroblast proliferation and tissue regeneration

Methods: Human foreskin fibroblasts were treated by Salmonella enterica LPS [100micro g] and extracts [5%w/w]. Tissues of male Balb/c mice were harvested at 1, 3 and 7 days for biochemical and histopathological evaluations. Effects of LPS and extracts on cell viability, Nitric oxide [NO], Cyclooxygenase-2 [COX-2] and Hydrogen peroxide H[2]O[2] levels were examined respectively by XTT assay and related kits

Results: Our pathological investigations for Camellia sinensis and LPS co-treated group indicated inflammatory cells on day 1 and fibroblast proliferation through wound area on day 3. After wound modelling the samples features were the same but with the difference in groups treated by LPS and Camellia sinensis extract which dermis and epidermis were seen. The Camellia sinensis extract and LPS co-treated wounds were showed low levels of H[2]O[2] and higher levels of NO compared to extract treated group [P<0.001]. Results illustrate a dose and time dependent significant difference in cell proliferation between groups

Conclusion: These results suggest that Camellia sinensis extract in combination with LPS may have potential of accelerating inflammatory phase of wound healing process by regulation of COX-2, NO and H[2]O[2] in skin fibroblast

[Study of oral administrated silymarin effects on UV-induced changes on albino guinea pigs skin]

Studies have shown that Silymarin is one of the infrequent herbal materials which have protective effects against sunburn, non melanoma skin cancer and immunosupression in oral administration. Easiness of use, low cost and availability of Silymarin can propose it for use in many topical sunscreen products. Regarding to the skin damages caused by the ultraviolet ray of sun and necessity to use of protective agents against mal effects of this ray, in this study the effects of oral use of Silymarin against effects of UV irradiation was evaluated. In this study 60 albino guinea pigs were selected and they divided in two equal groups. Animals in the experimental group administered 50 mg/kg Silymarin 20 days before and through the irradiation period orally. Control group received distilled water only. Then each group exposed to 180mj/cm2 UV ray. Finally clinical signs were noted and sampling was done from irradiated skin areas for histopathological evaluation. Histopathological and clinical findings showed that oral administration of Silymarin had protective effects against mal effects of UV irradiation on guinea pig skins. The results of this study can be incentive for introduction and use of this herbal agent in sun ray protective oral products because any topical sunscreen has not perfect protective effects against all UV irradiation spectrums

Study on effects of topical use of Silybum marianum extract [silymarin] on U.V. irradiated guinea pig skin

Regarding to the skin damages caused by the ultraviolet ray of sun and the need for protective materials against these mal effects, Silymarin was evaluated for the porpose. In this study, by histopathology studies and surveying the clinical symptoms the external use of an herbaceous [herbal] substance, Silymarin, in protecting the mal effects of ultraviolet ray has been evaluated. For the study 60 albino hamsters with same age and gender has been selected and they divided in two groups [each group 30 hamsters] randomly. The hair on the back of all animals shaved in an area about 2 square centimeters. The first group gets 9 miligram Silymarin with 20 micro liter acetone topically, and the second group gets only 20 micro liter acetone topically. Then both groups for 45 days exposed to ultra violet ray, 180 milijoule/cm[2] each day. The results of clinical and histopathologic surveys show that topical usage of Silymarin has considerable protective effects against the mal effects of ultra violet ray on skin and this can be a promise for using this herbaceous substance as a topical sun protects substance